Bio-rad ChromLab™ Software Manuale Utente

NGC™ Chromatography Systems and ChromLab™ SoftwareInstrument GuideVersion 3.03.0

viii | NGC Chromatography Systems and ChromLab SoftwareTable of ContentsOther Components . . . . . . . . . . . . . . . . . . . . . . . . . . . .

3 | Preparing the Instrument98 | NGC Chromatography Systems and ChromLab SoftwareChanging the Mixer BarrelThe mixer module is used to blend

Changing the Mixer BarrelInstrument Guide | 9 9To insert or change the mixer barrel To insert or change the mixer barrel, see Replacing the Mixer

3 | Preparing the Instrument100 | NGC Chromatography Systems and ChromLab SoftwareConnecting the Air Sensor ModuleThe air sensors enable the

Connecting the Air Sensor ModuleInstrument Guide | 101Air Sensor Tubing Length and Bubble SizeThe response of the air sensor module is highly depe

3 | Preparing the Instrument102 | NGC Chromatography Systems and ChromLab SoftwareAttaching and Activating Air SensorsThis section explains

Connecting the Air Sensor ModuleInstrument Guide | 1035. To connect an air sensor to the system pumps:a. Connect tubing from the buffer bottle or

3 | Preparing the Instrument104 | NGC Chromatography Systems and ChromLab SoftwareConnecting External Devices to the NGC InstrumentTip: If

Connecting External Devices to the NGC InstrumentInstrument Guide | 105Importing Analog Signals to the NGC InstrumentYou can import analog (voltag

3 | Preparing the Instrument106 | NGC Chromatography Systems and ChromLab Software3. Insert the other end of the cable into one of the perip

Connecting External Devices to the NGC InstrumentInstrument Guide | 107Connecting External Devices to the SIMWARNING! These procedures require y

Instrument Guide | i xTable of ContentsAppendix D Connecting the C-96 Autosampler to NGC Systems . . . . . . . . . . . . . . . . . . . . . . . .

3 | Preparing the Instrument108 | NGC Chromatography Systems and ChromLab Software3. Loosen all three screws on the top of the connector.Tip

Connecting External Devices to the NGC InstrumentInstrument Guide | 1099. On the analog output cable, determine the positive and negative wires.10

3 | Preparing the Instrument110 | NGC Chromatography Systems and ChromLab Software11. Insert the negative wire and one end of the jumper wir

Connecting External Devices to the NGC InstrumentInstrument Guide | 11113. Insert the connector into the SIM 1 or SIM 2 port on the back of the SI

3 | Preparing the Instrument112 | NGC Chromatography Systems and ChromLab SoftwareTo connect an external device to the DAC A and DIGITAL OUT

Connecting External Devices to the NGC InstrumentInstrument Guide | 1139. Insert the positive and ground wires into the appropriate terminals on t

3 | Preparing the Instrument114 | NGC Chromatography Systems and ChromLab SoftwareStarting the NGC InstrumentCaution: The NGC system runs u

Starting the NGC InstrumentInstrument Guide | 115Connecting the NGC System to ChromLab SoftwareNote: For information about installing ChromLab, s

3 | Preparing the Instrument116 | NGC Chromatography Systems and ChromLab SoftwareVerifying Plumbing with the Point-to-Plumb FeatureThe Poin

Verifying Plumbing with the Point-to-Plumb FeatureInstrument Guide | 117To verify the plumbing path 1. With both the Point-to-Plumb screen and the

x | NGC Chromatography Systems and ChromLab SoftwareTable of Contents

3 | Preparing the Instrument118 | NGC Chromatography Systems and ChromLab SoftwarePriming and Purging the SystemsThis section explains how t

Priming and Purging the SystemsInstrument Guide | 119Priming and Purging the NGC Quest SystemCaution: Before priming or purging the system, ensur

3 | Preparing the Instrument120 | NGC Chromatography Systems and ChromLab Software6. Remove the syringe from the priming port and discard th

Priming and Purging the SystemsInstrument Guide | 1215. In the fluidic scheme, touch or click the System Pump module to open its dialog box.6. In

3 | Preparing the Instrument122 | NGC Chromatography Systems and ChromLab SoftwarePriming and Purging the NGC Scout SystemPriming the NGC Sc

Priming and Purging the SystemsInstrument Guide | 1235. In the System Pump dialog box, select Priming on the Mode dropdown list.Note: When in Pri

3 | Preparing the Instrument124 | NGC Chromatography Systems and ChromLab SoftwarePurging the NGC Scout SystemTo purge the buffer blending v

Priming and Purging the SystemsInstrument Guide | 1253. To purge all lines of air bubbles and fill them with buffer:a. Click All.b. (Optional) Cha

3 | Preparing the Instrument126 | NGC Chromatography Systems and ChromLab SoftwarePriming and Purging the NGC Discover SystemPriming the NGC

Priming and Purging the SystemsInstrument Guide | 1274. In the fluidic scheme, touch or click the System Pump module to open its dialog box.5. In

Instrument Guide | 1 11 IntroductionThe NGC™ chromatography systems are preparative systems designed to rapidly automate the purification of biomo

3 | Preparing the Instrument128 | NGC Chromatography Systems and ChromLab Software7. Insert a 30 ml syringe into the priming port on system

Priming and Purging the SystemsInstrument Guide | 129To prime the buffer inlet valves1. Immerse the tubing for the inlet valves in their respectiv

3 | Preparing the Instrument130 | NGC Chromatography Systems and ChromLab Software8. Remove the syringe from the priming port and discard th

Priming and Purging the SystemsInstrument Guide | 131To prime the sample inlet valve (if available)1. Immerse the tubing for the inlet valve in th

3 | Preparing the Instrument132 | NGC Chromatography Systems and ChromLab Software5. Insert a 30 ml syringe into the sample pump’s priming p

Priming and Purging the SystemsInstrument Guide | 133Purging the NGC Discover SystemTo purge the buffer blending valve1. Open the System Pump dial

3 | Preparing the Instrument134 | NGC Chromatography Systems and ChromLab Software2. To purge an individual line of air bubbles and fill it

Priming and Purging the SystemsInstrument Guide | 135To purge the buffer inlet valves1. Open the System Pump dialog box and select Priming on the

3 | Preparing the Instrument136 | NGC Chromatography Systems and ChromLab SoftwareTo purge the sample pump1. In the fluidic scheme, touch or

Priming and Purging the SystemsInstrument Guide | 1373. Click Change Position to set the sample inject valve to Sample Pump Waste and close the di

1 | Introduction12 | NGC Chromatography Systems and ChromLab SoftwareThe NGC Chromatography SystemsThe NGC chromatography systems are availa

3 | Preparing the Instrument138 | NGC Chromatography Systems and ChromLab Software2. In the Sample Pump dialog box, do one of the following:

Preparing the System and Sample Pump Piston Washing SystemsInstrument Guide | 139Preparing the System and Sample Pump Piston Washing SystemsNote:

3 | Preparing the Instrument140 | NGC Chromatography Systems and ChromLab SoftwarePreparing the ColumnsBefore a method can be run, the colum

Preparing the ColumnsInstrument Guide | 141Connecting Column Clamps to the NGC InstrumentThe NGC instrument has indented metallic strips on both t

3 | Preparing the Instrument142 | NGC Chromatography Systems and ChromLab SoftwareConnecting Tubing to the ColumnsNote: Ensure that the sys

Preparing the ColumnsInstrument Guide | 143Washing the ColumnsCleaning a column requires access to ChromLab. You can perform this task from the Ch

3 | Preparing the Instrument144 | NGC Chromatography Systems and ChromLab Softwaree. Click Start.4. Run the buffer solution through the colu

Calibrating the NGC InstrumentInstrument Guide | 145Calibrating the NGC Instrument The NGC systems include integrated pressure and temperature sen

3 | Preparing the Instrument146 | NGC Chromatography Systems and ChromLab SoftwareCalibrating the Pump Flow RatePrime and purge the system p

Calibrating the NGC InstrumentInstrument Guide | 1473. Follow the steps in the Calibration dialog box.4. Verify that Status (at the bottom of the

Main FeaturesInstrument Guide | 1 3 NGC Discover Pro chromatography system includes All modules on the NGC Discover systemNote: Only the multi-

3 | Preparing the Instrument148 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 149A Maintaining the InstrumentRegular cleaning and maintenance ensures that your NGC™ system continues to perform optimally. T

A | Maintaining the Instrument150 | NGC Chromatography Systems and ChromLab SoftwareCleaning the Outer Surfaces of the NGC InstrumentDuring

Storing the NGC InstrumentInstrument Guide | 151Storing the NGC InstrumentTip: You can use the System CIP/Storage phase in ChromLab to store the

A | Maintaining the Instrument152 | NGC Chromatography Systems and ChromLab SoftwareRecommended Maintenance ScheduleTable 14 lists recommen

Recommended Maintenance ScheduleInstrument Guide | 153YearlySample pump seals and check valveReplace the seals and check valves using the pump hea

A | Maintaining the Instrument154 | NGC Chromatography Systems and ChromLab SoftwareAs NeededTubing and connectors Replace all tubing and co

Recommended Cleaning and Storage SolutionsInstrument Guide | 155Recommended Cleaning and Storage SolutionsTable 15 lists the recommended cleaning

A | Maintaining the Instrument156 | NGC Chromatography Systems and ChromLab SoftwareSystem and Sample PumpsThe NGC system’s gradient pump sy

System and Sample PumpsInstrument Guide | 157Pump Head Assembly LEGEND1 Outlet check valve housing 2 Pump head small washer3 Pump head seal 4 Inle

1 | Introduction14 | NGC Chromatography Systems and ChromLab Software Extend the preconfigured systems with additional valves for buffers,

A | Maintaining the Instrument158 | NGC Chromatography Systems and ChromLab SoftwarePiston Wash Assembly LEGEND1 Fitting 2 Piston base3 Pist

System and Sample PumpsInstrument Guide | 159Disassembling the PumpsThe system and sample pumps can be disassembled to clean or replace their comp

A | Maintaining the Instrument160 | NGC Chromatography Systems and ChromLab Software3. Use the 3/16" hex key that is provided in the fi

System and Sample PumpsInstrument Guide | 1615. Locate the wash housing O-ring and the pump head small washer. If you do not see them on the separ

A | Maintaining the Instrument162 | NGC Chromatography Systems and ChromLab Software9. (Optional) Inspect and clean the piston.Note: To rep

System and Sample PumpsInstrument Guide | 1632. Unscrew each IN check valve housing and pull it down out of the pump head.You should have removed

A | Maintaining the Instrument164 | NGC Chromatography Systems and ChromLab Software3. Insert two check valve capsules into each IN check va

System and Sample PumpsInstrument Guide | 165To install the pump head check valve assemblies1. To determine the inlet and outlet pump head housing

A | Maintaining the Instrument166 | NGC Chromatography Systems and ChromLab Software4. Insert an OUT check valve assembly into the open port

System and Sample PumpsInstrument Guide | 167To replace the pump head seal1. Insert the seal removal end of the small seal tool in the seal cavity

Site RequirementsInstrument Guide | 1 5EnvironmentThe NGC instruments are designed to work within temperatures of 4–40°C.The small footprint makes

A | Maintaining the Instrument168 | NGC Chromatography Systems and ChromLab Software6. Remove the seal tool and inspect the pump head to ver

System and Sample PumpsInstrument Guide | 169Removing the Wash HousingTo remove the wash housing1. Complete the steps in the section Removing the

A | Maintaining the Instrument170 | NGC Chromatography Systems and ChromLab SoftwareRemoving the PistonCaution: Use extreme caution when re

System and Sample PumpsInstrument Guide | 1715. Gently grasp the piston by its metal base and carefully lift the piston straight up to dislodge it

A | Maintaining the Instrument172 | NGC Chromatography Systems and ChromLab Software6. Rinse the piston and examine it for damage.7. Store t

System and Sample PumpsInstrument Guide | 173To replace the wash housing check valves1. Identify the left and right wash housings. The wash syste

A | Maintaining the Instrument174 | NGC Chromatography Systems and ChromLab SoftwareTo identify the left and right housings after you remove

System and Sample PumpsInstrument Guide | 175Reassembling the PumpsThis section explains how to Insert the piston Install the wash housing Inst

A | Maintaining the Instrument176 | NGC Chromatography Systems and ChromLab SoftwareInstalling the Wash HousingBefore installing the wash ho

System and Sample PumpsInstrument Guide | 1774. Position the left wash housing with its arrow pointing up and carefully slide it into place over t

1 | Introduction16 | NGC Chromatography Systems and ChromLab SoftwareSafety RequirementsThis section explains the different safety alerts in

A | Maintaining the Instrument178 | NGC Chromatography Systems and ChromLab SoftwareInstalling the Pump HeadsNote: When installing new pump

System and Sample PumpsInstrument Guide | 1796. Identify the pump head assemblies. To identify the left and right pump heads, position both pump h

A | Maintaining the Instrument180 | NGC Chromatography Systems and ChromLab Software8. Push on the center of the pump head to seat it firmly

System and Sample PumpsInstrument Guide | 181Replacing the Priming Manifold Check ValveThe priming manifold check valve has one check valve capsul

A | Maintaining the Instrument182 | NGC Chromatography Systems and ChromLab Software2. Using a pair of tweezers, remove the check valve caps

System and Sample PumpsInstrument Guide | 1835. Using the tweezers, insert the new check valve capsule into the priming manifold outlet port.Note:

A | Maintaining the Instrument184 | NGC Chromatography Systems and ChromLab Software6. Carefully insert the OUT check valve housing in the p

System and Sample PumpsInstrument Guide | 185Conditioning New SealsNew seals should be conditioned before use. To condition the seals, run the pum

A | Maintaining the Instrument186 | NGC Chromatography Systems and ChromLab SoftwareMixerReplacing the Mixer ComponentsThe NGC system’s mixi

MixerInstrument Guide | 187

Finding Out MoreInstrument Guide | 1 7Finding Out MoreAfter you install NGC documentation from the NGC Chromatography Systems Documentation DVD, y

A | Maintaining the Instrument188 | NGC Chromatography Systems and ChromLab SoftwareDisassembling the MixerUse this procedure to replace any

MixerInstrument Guide | 1893. Twist the mixer assembly clockwise approximately 1/8 turn and lift it upward to remove it from the socket.4. Tilt th

A | Maintaining the Instrument190 | NGC Chromatography Systems and ChromLab Software6. Separate the mixer top from the mixer base and barrel

MixerInstrument Guide | 191To change the O-rings1. Locate the O-ring in the groove on the mixer base and the mixer barrel extension (if used).2. W

A | Maintaining the Instrument192 | NGC Chromatography Systems and ChromLab SoftwareTo replace or exchange the mixer barrelTip: If you are

MixerInstrument Guide | 193To clean the mixer body1. Wipe the inside of the mixer body with a lint-free towel.2. Soak and sonicate the following c

A | Maintaining the Instrument194 | NGC Chromatography Systems and ChromLab SoftwareReassembling the MixerTip: To reinsert the magnetic st

MixerInstrument Guide | 1953. Using the hex key, firmly tighten the hex bolts. Alternate by tightening opposite bolts (tighten Bolt 1, then Bolt 3

A | Maintaining the Instrument196 | NGC Chromatography Systems and ChromLab SoftwareThe DetectorsReplacing the UV Flow CellThe NGC systems s

The DetectorsInstrument Guide | 1974. Firmly grasp the bolts on the flow cell and lift the flow cell from its socket.Multi-Wavelength UV/Vis Flow

1 | Introduction18 | NGC Chromatography Systems and ChromLab Software

A | Maintaining the Instrument198 | NGC Chromatography Systems and ChromLab Software5. Verify that the square gasket is removed with the flo

The DetectorsInstrument Guide | 1997. Locate the flow cell. The flow cell should include Two bolts One square rubber gasket8. Place the flow cel

A | Maintaining the Instrument200 | NGC Chromatography Systems and ChromLab Software9. Tighten the bolts. If necessary, use the screwdriver.

The DetectorsInstrument Guide | 201Replacing the Single-Wavelength UV Detector LEDThis section explains how to replace the LED on the single-wavel

A | Maintaining the Instrument202 | NGC Chromatography Systems and ChromLab Software

The DetectorsInstrument Guide | 203Tip: The door is tethered to the casing. It will hang to the side while you replace the lamps.

A | Maintaining the Instrument204 | NGC Chromatography Systems and ChromLab SoftwareYou see the LED module with three thumbscrews. The LED i

The DetectorsInstrument Guide | 2056. Loosen the left thumbscrews, alternating one turn on the top left screw then one turn on the bottom left scr

A | Maintaining the Instrument206 | NGC Chromatography Systems and ChromLab SoftwareThe following image shows the LED housing without the LE

The DetectorsInstrument Guide | 20710. Align the thumbscrews of the replacement LED with the holes on the housing and carefully insert the LED int

Instrument Guide | 1 92 The NGC InstrumentThe NGC™ instrument ships preassembled with the components necessary to perform gradient separations. Th

A | Maintaining the Instrument208 | NGC Chromatography Systems and ChromLab SoftwareResetting the Lamp Time DetailsThe system tracks the lam

The DetectorsInstrument Guide | 209Replacing the Multi-Wavelength UV/Vis Detector Lamps This section explains how to replace the deuterium lamp or

A | Maintaining the Instrument210 | NGC Chromatography Systems and ChromLab SoftwareThe deuterium and tungsten lamps are both accessible.Tip

The DetectorsInstrument Guide | 2116. Remove the lamp:WARNING! Use caution when removing the lamps. The lamps might be hot to the touch after th

A | Maintaining the Instrument212 | NGC Chromatography Systems and ChromLab Softwarec. With a 2.5 mm hex key, remove the screws on the top a

The DetectorsInstrument Guide | 21311. Insert the cable plug and tighten the collar.Tip: The deuterium lamp plug has seven connector pins, the t

A | Maintaining the Instrument214 | NGC Chromatography Systems and ChromLab SoftwareReplacing the Conductivity MonitorThe images in this sec

The DetectorsInstrument Guide | 2154. Firmly grasp the detector and pull it toward you to detach it.5. Dispose of the detector in an appropriate w

A | Maintaining the Instrument216 | NGC Chromatography Systems and ChromLab Software8. Gently push the detector onto the connector pins on t

pH ProbeInstrument Guide | 217pH ProbeStoring the pH ElectrodeWhen not in use, store the pH electrode in storage solution. See Table 15 on page

2 | The NGC Instrument20 | NGC Chromatography Systems and ChromLab SoftwareThe NGC Instrument IllustratedThe illustrations in this section d

A | Maintaining the Instrument218 | NGC Chromatography Systems and ChromLab SoftwareTo remove lipophilic organic contaminants Immerse the e

pH ProbeInstrument Guide | 2193. Loosen the black collar on the pH probe.4. To remove the pH probe, lift the probe out of the pH flow cell.Dispose

A | Maintaining the Instrument220 | NGC Chromatography Systems and ChromLab SoftwareOther ComponentsAttaching an Expansion Tier to the NGC I

Other ComponentsInstrument Guide | 221Tip: The buffer tray can remain inside the top.4. Locate the package that contains the expansion tier and

A | Maintaining the Instrument222 | NGC Chromatography Systems and ChromLab Software7. Locate the connector cable that ships with the expans

Other ComponentsInstrument Guide | 223For example:

A | Maintaining the Instrument224 | NGC Chromatography Systems and ChromLab Software9. Firmly press the clips inward and insert the appropri

Other ComponentsInstrument Guide | 22510. Insert the other connector into the lower tier’s connector port.11. Place the top, with the buffer tray,

A | Maintaining the Instrument226 | NGC Chromatography Systems and ChromLab Software13. Perform the following tasks:Task: Refer to the secti

Other ComponentsInstrument Guide | 227Replacing or Repositioning Modules on the NGC InstrumentsWARNING! Disconnect power to the NGC instrument b

This page is intentionally blank.The NGC Instrument IllustratedInstrument Guide | 2 1

A | Maintaining the Instrument228 | NGC Chromatography Systems and ChromLab SoftwareThe following image shows an empty bay.5. Store the modu

Other ComponentsInstrument Guide | 2298. The frame of the bay has tracks on the top and bottom of the right side. The module has guide rails on th

A | Maintaining the Instrument230 | NGC Chromatography Systems and ChromLab Software13. Perform the following tasks:Task: Refer to the secti

Other ComponentsInstrument Guide | 231Converting Bays to Fit ModulesSome modules fit into single-wide bays while others require double-wide bays (

A | Maintaining the Instrument232 | NGC Chromatography Systems and ChromLab SoftwareTo convert a single-wide bay to a double-wide bay1. Comp

Other ComponentsInstrument Guide | 233To convert a double-wide bay to a single-wide bay1. Locate a divider.2. Complete steps 1–5 in the section To

A | Maintaining the Instrument234 | NGC Chromatography Systems and ChromLab SoftwareCutting Replacement TubingTip: Use the tubing cutter p

Other ComponentsInstrument Guide | 2354. Slide the fitting nut, lock ring, and the ferrule, in that order, onto the tubing.Note: The stainless st

A | Maintaining the Instrument236 | NGC Chromatography Systems and ChromLab SoftwareInstalling the Backpressure RegulatorThe NGC systems shi

Other ComponentsInstrument Guide | 237To remove the backpressure regulator1. Disconnect the tubing from the top and bottom of the installed backpr

2 | The NGC Instrument22 | NGC Chromatography Systems and ChromLab SoftwareFront View

A | Maintaining the Instrument238 | NGC Chromatography Systems and ChromLab SoftwareRepositioning the Touch ScreenThis procedure explains ho

Other ComponentsInstrument Guide | 2394. Remove the side mount cover from the side of the instrument on which you plan to attach the touch screen.

A | Maintaining the Instrument240 | NGC Chromatography Systems and ChromLab Software6. Remove the display cable from the side and rear cable

Other ComponentsInstrument Guide | 2418. Lift the touch screen display off the mounting post.9. Place the touch screen monitor face down on a clea

A | Maintaining the Instrument242 | NGC Chromatography Systems and ChromLab Software11. Using the 7/64 hex key provided in the accessories k

Other ComponentsInstrument Guide | 24314. Turn the pivot arm assembly around 180° so that the mounting assembly is now in your left hand and inser

A | Maintaining the Instrument244 | NGC Chromatography Systems and ChromLab Software20. Route the display cable through the side and rear ca

Instrument Guide | 245B Troubleshooting the InstrumentThis appendix lists potential problems and suggested solutions for the NGC™ chromatography s

B | Troubleshooting the Instrument246 | NGC Chromatography Systems and ChromLab SoftwareThe pumps do not deliver the correct flow rate (cont

System and Sample PumpsInstrument Guide | 247The pumps do not deliver the correct flow rate (continued).Air bubbles might be trapped in the pump h

The NGC Instrument IllustratedInstrument Guide | 2 3LEGEND1 Touch screen monitor 2 Sample pump3 Sample inlet valve 4 Buffer tray5 Empty bay, cover

B | Troubleshooting the Instrument248 | NGC Chromatography Systems and ChromLab SoftwareThe pumps do not deliver the correct flow rate (cont

System and Sample PumpsInstrument Guide | 249Liquid continuously leaks from the pump head washout drain trough (continued).The check valves might

B | Troubleshooting the Instrument250 | NGC Chromatography Systems and ChromLab SoftwareThe backpressure drops to zero or is much lower than

System and Sample PumpsInstrument Guide | 251Backpressure values are higher or lower than expected. The flow rate might have changed. In ChromLab,

B | Troubleshooting the Instrument252 | NGC Chromatography Systems and ChromLab SoftwareBackpressure values are higher or lower than expecte

UV or UV/Vis DetectorInstrument Guide | 253UV or UV/Vis DetectorSymptom Possible Cause Possible SolutionThe UV baseline is unstable or noisy.The U

B | Troubleshooting the Instrument254 | NGC Chromatography Systems and ChromLab SoftwareThe UV baseline is unstable or noisy (continued).The

UV or UV/Vis DetectorInstrument Guide | 255Baseline noise continues when pumps are turned off.External environmental influences can cause noise sp

B | Troubleshooting the Instrument256 | NGC Chromatography Systems and ChromLab SoftwareThe baseline is drifting. The eluant is nonhomogeneo

UV or UV/Vis DetectorInstrument Guide | 257Sample trace values are lower than expected.The lamps might be nearing the end of their usable life.Che

2 | The NGC Instrument24 | NGC Chromatography Systems and ChromLab SoftwareBack ViewLEGEND1 Cable connector port/hand grip 2 Cable connector

B | Troubleshooting the Instrument258 | NGC Chromatography Systems and ChromLab SoftwareConductivity MonitorSymptom Possible Cause Possible

pH ProbeInstrument Guide | 259pH ProbeSymptom Possible Cause Possible SolutionThe pH readings are higher or lower than expected.The pH probe might

B | Troubleshooting the Instrument260 | NGC Chromatography Systems and ChromLab SoftwareBuffer Blending ValveColumn Switching ValveSymptom P

External DevicesInstrument Guide | 261External DevicesSymptom Possible Cause Possible SolutionUnable to zero an external device connected to SIM 1

B | Troubleshooting the Instrument262 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 263C Plumbing the SystemWith the exception of the inlet tubing, waste lines, and column, the NGC™ systems ship preassembled and

C | Plumbing the System264 | NGC Chromatography Systems and ChromLab SoftwarePlumbing NGC SystemsSeveral plumbing tasks are required for all

Plumbing NGC SystemsInstrument Guide | 265Plumbing All NGC SystemsTable 19 lists the tasks for plumbing all NGC systems. The tasks are explained

C | Plumbing the System266 | NGC Chromatography Systems and ChromLab SoftwareSystem Pumps Wash FlowSample Pump Wash Flow

Plumbing NGC SystemsInstrument Guide | 267To plumb and prime the system pump wash lines1. Mount a tube holder to the right or left side of the ins

The NGC Instrument IllustratedInstrument Guide | 2 5Right Side ViewLEGEND1 Drain hole 2 Peripheral port 3 Peripheral ports 4 USB ports5 Soft power

C | Plumbing the System268 | NGC Chromatography Systems and ChromLab Software8. Attach a 30 ml syringe to the 1/4-28 female to male luer ada

Plumbing NGC SystemsInstrument Guide | 269Plumbing the Sample Inject ValveTo plumb the sample inject valve1. Connect waste tubing to waste ports W

C | Plumbing the System270 | NGC Chromatography Systems and ChromLab SoftwareAttaching the Backpressure RegulatorIf your column requires a h

Plumbing NGC SystemsInstrument Guide | 271Plumbing the NGC Quest SystemTable 20 lists the tasks for plumbing the NGC Quest™ system. The tasks are

C | Plumbing the System272 | NGC Chromatography Systems and ChromLab SoftwareNGC Quest Point-to-Plumb Connections

Plumbing NGC SystemsInstrument Guide | 273To plumb the system pumps1. Connect inlet tubing to the inlet manifold of system pump A.2. Connect inlet

C | Plumbing the System274 | NGC Chromatography Systems and ChromLab SoftwarePlumbing the NGC Scout SystemTable 21 lists the tasks for plum

Plumbing NGC SystemsInstrument Guide | 275NGC Scout Point-to-Plumb Connections

C | Plumbing the System276 | NGC Chromatography Systems and ChromLab SoftwareTo plumb the buffer blending valve Connect the inlet tubing to

Plumbing NGC SystemsInstrument Guide | 2773. Immerse the open end of the waste tubing in the waste container.4. Connect Tube #2 from the 40 psi ba

2 | The NGC Instrument26 | NGC Chromatography Systems and ChromLab SoftwareLeft Side ViewLEGEND1 Buffer tray 2 Drain hole3 Touch screen cabl

C | Plumbing the System278 | NGC Chromatography Systems and ChromLab SoftwarePlumbing the NGC Discover SystemTable 22 lists the tasks for p

Plumbing NGC SystemsInstrument Guide | 279NGC Discover Point-to-Plumb Connections

C | Plumbing the System280 | NGC Chromatography Systems and ChromLab SoftwareTo plumb the buffer blending valve Connect the inlet tubing to

Plumbing NGC SystemsInstrument Guide | 281To plumb the buffer inlet valves1. Connect tubing from Outlet A on the buffer blending valve to port 8/B

C | Plumbing the System282 | NGC Chromatography Systems and ChromLab SoftwareTo plumb the pH valve1. Connect the pump head wash priming tubi

Plumbing NGC SystemsInstrument Guide | 283Plumbing the NGC Discover Pro SystemTable 23 lists the tasks for plumbing the NGC Discover Pro system.

C | Plumbing the System284 | NGC Chromatography Systems and ChromLab SoftwareNGC Discover Pro Point-to-Plumb Connections

Plumbing NGC SystemsInstrument Guide | 285Plumbing Sample Inlet ValvesEach sample inlet valve module includes all necessary tubing. The inlet tubi

C | Plumbing the System286 | NGC Chromatography Systems and ChromLab Software2. If your system has two sample inlet valves:a. Connect tubing

Plumbing NGC SystemsInstrument Guide | 287b. (Optional) Do one of the following: Connect Tube #4 from port 1 to the Common inlet port on the BioF

The NGC Instrument IllustratedInstrument Guide | 2 7Liquid Flow Path of the NGC Discover Pro SystemAt startup, ChromLab displays a default fluidic

C | Plumbing the System288 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 289D Connecting the C-96 Autosampler to NGC SystemsUsing the C-96 Autosampler with NGC SystemsThe C-96 autosampler enhances NGC

D | Connecting the C-96 Autosampler to NGC Systems290 | NGC Chromatography Systems and ChromLab Software5. Locate the two cables that conn

Using the C-96 Autosampler with NGC SystemsInstrument Guide | 2918. On the back of the autosampler:a. Insert the 15-pin cable into the left (P1 RE

D | Connecting the C-96 Autosampler to NGC Systems292 | NGC Chromatography Systems and ChromLab SoftwarePlumbing and Priming the C-96 Auto

Using the C-96 Autosampler with NGC SystemsInstrument Guide | 293To prime and purge the C-96 autosamplerNote: Before priming the autosampler, ens

D | Connecting the C-96 Autosampler to NGC Systems294 | NGC Chromatography Systems and ChromLab Software7. In the fluidic scheme, touch or

Using the C-96 Autosampler with NGC SystemsInstrument Guide | 295Setting Up the C-96 Autosampler for Use in ChromLab MethodsThis section briefly e

D | Connecting the C-96 Autosampler to NGC Systems296 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 297E Regulatory InformationThe NGC™ instrument has been tested and found to be in compliance with all applicable requirements o

NGC™ Chromatography Systems and ChromLab™ SoftwareInstrument GuideVersion 3.0

2 | The NGC Instrument28 | NGC Chromatography Systems and ChromLab SoftwarePumpsThe NGC systems can have up to three high-precision pumps: t

E | Regulatory Information298 | NGC Chromatography Systems and ChromLab SoftwareSample Loop PSI Ratings for U.S. and CanadaThe following pre

FCC Warning and NotesInstrument Guide | 299FCC Warning and NotesThe NGC instrument complies with part 15 of the FCC Rules. Operation is subject to

E | Regulatory Information300 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 301F NGC Chromatography Systems Catalog NumbersTable 25. NGC™ chromatography systems catalog numbersSystem Catalog numberNGC

F | NGC Chromatography Systems Catalog Numbers302 | NGC Chromatography Systems and ChromLab Software

Life ScienceGroup14-0273 0114 Sig 121310026253 Rev C US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

PumpsInstrument Guide | 2 9System PumpsThe system pumps perform isocratic or gradient elution at the specified flow rates.LEGEND1 Pump flow status

2 | The NGC Instrument30 | NGC Chromatography Systems and ChromLab SoftwareDetailed AttributesThe NGC systems include two system pumps: pump

PumpsInstrument Guide | 3 1Each system pump module has an emergency Pause button and a Purge button. Pressing the emergency Pause button on eithe

2 | The NGC Instrument32 | NGC Chromatography Systems and ChromLab SoftwarePiston Wash SystemThe pumps on the NGC system automatically rinse

PumpsInstrument Guide | 3 3Sample PumpThe sample pump is used to automatically load large sample volumes onto a column or to automatically fill la

2 | The NGC Instrument34 | NGC Chromatography Systems and ChromLab SoftwareDetailed Attributes Flow rate delivery: 0.01–100 ml/min Pressur

PumpsInstrument Guide | 3 5Tip: You can pause the system through ChromLab using either the touch screen or the ChromLab computer. You can also s

2 | The NGC Instrument36 | NGC Chromatography Systems and ChromLab SoftwareValvesAll NGC systems include a sample inject valve. Your system

ValvesInstrument Guide | 3 7Sample Inject ValveThe sample inject valve enables the system to load a specific, predetermined volume of sample onto

Bio-Rad Technical Support DepartmentThe Bio-Rad Technical Support department in the U.S. is open Monday through Friday, 5:00 AM to 5:00 PM, Pacific Ti

2 | The NGC Instrument38 | NGC Chromatography Systems and ChromLab SoftwarePorts on the Sample Inject Valve Port FunctionInject Manually loa

ValvesInstrument Guide | 3 9Sample Inject Valve LEDsThe sample inject valve module is fitted with LEDs. Solid green LEDs indicate the flow is fro

2 | The NGC Instrument40 | NGC Chromatography Systems and ChromLab SoftwareValve Position ExplanationManual Load Loop/System Pump to Column

ValvesInstrument Guide | 4 1Sample Pump Direct Inject/System Pump Waste Directs system pump flow to waste (W2) Directs sample pump flow directly

2 | The NGC Instrument42 | NGC Chromatography Systems and ChromLab SoftwareBuffer Blending Valve The buffer blending valve is used for autom

ValvesInstrument Guide | 4 3Detailed Attributes Operating pressure up to 15 psi

2 | The NGC Instrument44 | NGC Chromatography Systems and ChromLab SoftwarePorts on the Buffer Blending Valve Buffer Blending Valve LEDsThe

ValvesInstrument Guide | 4 5How the Buffer Blending Valve WorksThe buffer blending valve operates in two modes: buffer blending and gradient.Buffe

2 | The NGC Instrument46 | NGC Chromatography Systems and ChromLab SoftwareInlet ValvesThe inlet valves automate the use of multiple buffers

ValvesInstrument Guide | 4 7Inlet ValveDetailed Attributes Maximum operating pressure: 500 psi  Optional air sensors indicate the end of buffer

Instrument Guide | iiiTable of ContentsChapter 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .11The N

2 | The NGC Instrument48 | NGC Chromatography Systems and ChromLab SoftwarePorts on the Inlet ValvesBuffer Inlet ValveSample Inlet ValvePort

ValvesInstrument Guide | 4 9Inlet Valve LEDsThe inlet valve modules are fitted with LEDs. Solid green LEDs indicate the flow is from the system p

2 | The NGC Instrument50 | NGC Chromatography Systems and ChromLab SoftwareHow the Inlet Valves WorkThe function of an inlet valve is determ

ValvesInstrument Guide | 5 1Outlet ValveThe outlet valve enables the system to direct the flow to the BioFrac™ fraction collector, to waste, or to

2 | The NGC Instrument52 | NGC Chromatography Systems and ChromLab SoftwareDetailed Attributes Maximum operating pressure is 500 psiPorts o

ValvesInstrument Guide | 5 3Outlet Valve LEDsThe outlet valve modules are fitted with LEDs. Solid green LEDs indicate the flow is from the system

2 | The NGC Instrument54 | NGC Chromatography Systems and ChromLab SoftwareHow the Outlet Valve WorksThe NGC system supports the use of up t

ValvesInstrument Guide | 5 5Column Switching ValveThe NGC system supports up to three column switching valves. Each column switching valve is used

2 | The NGC Instrument56 | NGC Chromatography Systems and ChromLab SoftwareDetailed Attributes Maximum operating pressure: 3,650 psi  Dual

ValvesInstrument Guide | 5 7Ports on the Column Switching Valve Port FunctionTop 1–5 Ports to connect to the top of columnsBottom 1–5 Ports

iv | NGC Chromatography Systems and ChromLab SoftwareTable of ContentsHow the Sample Pump Works . . . . . . . . . . . . . . . . . . . . . . . .

2 | The NGC Instrument58 | NGC Chromatography Systems and ChromLab SoftwareColumn Switching Valve LEDsThe column switching valve modules are

ValvesInstrument Guide | 5 9The section Flow Paths of the Column Switching Valve explains the specific flow paths.The column switching valve has i

2 | The NGC Instrument60 | NGC Chromatography Systems and ChromLab Software Select Bypass to bypass the flow past all ports. Select Revers

MixerInstrument Guide | 6 1Mixer The mixer module consists of a mixer assembly and an integrated system pressure sensor. This module homogenizes t

2 | The NGC Instrument62 | NGC Chromatography Systems and ChromLab SoftwareDuring operation, the system pressure sensor continuously measure

MixerInstrument Guide | 6 3Detailed AttributesThe mixer is composed of a base motor, a stirring plate, and a hollow mixer barrel in which the buff

2 | The NGC Instrument64 | NGC Chromatography Systems and ChromLab SoftwareMixer LEDsThe mixer module is fitted with LEDs. A left green LED

DetectorsInstrument Guide | 6 5Single-Wavelength UV Detector and Conductivity MonitorMulti-Wavelength UV Detector and Conductivity Monitor

2 | The NGC Instrument66 | NGC Chromatography Systems and ChromLab SoftwareSingle-Wavelength UV DetectorDetailed Attributes Two integrated

DetectorsInstrument Guide | 6 7 Monitors UV absorbance one wavelength at a time 255 nm  280 nm (default)Tip: Use ChromLab to change the absor

Instrument Guide | vTable of ContentsMulti-Wavelength UV/Vis Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68Conductiv

2 | The NGC Instrument68 | NGC Chromatography Systems and ChromLab SoftwareMulti-Wavelength UV/Vis DetectorDetailed Attributes Contains one

DetectorsInstrument Guide | 6 9 Monitors up to four wavelengths in the UV/Vis range of 190–800 nmTip: Use ChromLab to change the absorbance wav

2 | The NGC Instrument70 | NGC Chromatography Systems and ChromLab SoftwareHow They WorkUV DetectorsChromLab recognizes the connected optic

DetectorsInstrument Guide | 7 1The Backpressure RegulatorA backpressure regulator can be added to the flow path to generate backpressure and preve

2 | The NGC Instrument72 | NGC Chromatography Systems and ChromLab SoftwarepH DetectorThe pH detector includes an integrated flow cell in wh

DetectorsInstrument Guide | 7 3Detailed Attributes Flow cell volume: 90 μl (200 μl including internal valve passageways) Electrode maximum opera

2 | The NGC Instrument74 | NGC Chromatography Systems and ChromLab SoftwareHow the pH Detector WorksThe integrated pH electrode enables inli

DetectorsInstrument Guide | 7 5Changing the Flow Paths of the pH ValveDuring a manual run, you can change the flow path of the pH valve by accessi

2 | The NGC Instrument76 | NGC Chromatography Systems and ChromLab SoftwareTubing, Loops, Columns, and FittingsThis section lists the access

Tubing, Loops, Columns, and FittingsInstrument Guide | 7 7Sample Injection LoopsColumnsThe NGC chromatography systems support columns for various

vi | NGC Chromatography Systems and ChromLab SoftwareTable of ContentsConnecting the Sample Injection Loops . . . . . . . . . . . . . . . . . .

2 | The NGC Instrument78 | NGC Chromatography Systems and ChromLab SoftwareFittingsThe NGC chromatography systems ship with 10/32 fittings f

LEDs and LED ScreensInstrument Guide | 7 9LEDs and LED ScreensThe modules on the NGC system are fitted with light emitting diodes (LEDs) that are

2 | The NGC Instrument80 | NGC Chromatography Systems and ChromLab SoftwareSensorsAir SensorsThe NGC chromatography systems can be fitted wi

SensorsInstrument Guide | 8 1The air sensors are inserted into ports on the air sensor module. ChromLab detects the presence or absence of air sen

2 | The NGC Instrument82 | NGC Chromatography Systems and ChromLab SoftwareAir Sensor LEDsThe air sensor module is fitted with LEDs. Green

SensorsInstrument Guide | 8 3Pressure SensorsThe NGC systems can have up to four integrated pressure sensors: System pressure sensor — located in

2 | The NGC Instrument84 | NGC Chromatography Systems and ChromLab SoftwareNGC Touch ScreenThe NGC system’s touch screen is the main interfa

Buffer TrayInstrument Guide | 8 5Buffer TrayThe buffer tray on the top of the instrument can accommodate up to six 1 L bottles.Note: Ensure that

2 | The NGC Instrument86 | NGC Chromatography Systems and ChromLab SoftwareProduct ConfigurationsThe NGC chromatography system is available

Product ConfigurationsInstrument Guide | 8 7Fourth expansion tierSample inlet valve 1Outlet valve 1*Only the multi-wavelength UV/Vis detector i

Instrument Guide | viiTable of ContentsAppendix A Maintaining the Instrument . . . . . . . . . . . . . . . . . . . . 149Cleaning the Outer Surfa

2 | The NGC Instrument88 | NGC Chromatography Systems and ChromLab Software

Instrument Guide | 8 93 Preparing the InstrumentThe NGC™ instruments ship preassembled with the modules necessary to perform chromatographic separ

3 | Preparing the Instrument90 | NGC Chromatography Systems and ChromLab SoftwareModule ReviewThe NGC systems are shipped in one of three st

Module ReviewInstrument Guide | 9 1NGC Discover™ Chromatography SystemNGC Discover Pro Chromatography System

3 | Preparing the Instrument92 | NGC Chromatography Systems and ChromLab SoftwareNGC Systems Accessories KitEach NGC system ships with an ac

NGC Systems Accessories KitInstrument Guide | 9 3NGC Scout Accessories KitThe kit for the NGC Scout system includes all the accessories in the NGC

3 | Preparing the Instrument94 | NGC Chromatography Systems and ChromLab SoftwarePreparing the Buffer and Waste BottlesAfter you plumb the i

Connecting the Sample Injection LoopsInstrument Guide | 9 5Connecting the Sample Injection LoopsThe NGC chromatography systems support a variety o

3 | Preparing the Instrument96 | NGC Chromatography Systems and ChromLab SoftwareInserting Check Valves into the Sample Inject ValveAll NGC

Preparing the DetectorsInstrument Guide | 9 74. Connect the tubing to the check valves.Preparing the DetectorsThe NGC systems ship with the conduc