Bio-rad BioLogic LP System Manuale Utente Scaricare il pdf

The Gradient Mixer proportions two buffers as commanded by either the BioLogic LP Controller or the

Econo Gradient Pump and mixes the buffers in a dynamic mixing chamber to form a gradient. This

valve may be used with either the BioLogic LP system or the Econo Gradient Pump; both applications

are shown below.

Figure 1. Gradient Mixer

I Use with the BioLogic LP

Plumbing and Operation of the Gradient Mixer

1.To attach the Gradient Mixer to the system rack:

a.Ensure that the clamping screw is loose.

b.Clip the attaching bracket to a convenient place on the rack.

c.Tighten the clamping screw.

d.Connect the signal cable from the Gradient Mixer to the port marked “Gradient Mixer” on the

rear of the BioLogic LP Controller.

2.Plumb the Gradient Mixer’s inlet ports to the solvent reservoirs.

Using barb-to-male luers, connect an appropriate length of tubing between buffer ports A and B to

their respective solvent reservoirs (see Figure 2.) Match the appropriate luer connector with tubing

ID, and ensure a firm connection but do not overtighten. Similarly, connect the gradient mixer to

either port A/B of the LP Buffer Select Valve or directly to the pump inlet if the Buffer Select valve

is not used.

3.If a Method includes gradient formation, then the Gradient Mixer’s proportioning valve will

automatically toggle between the two positions to create the gradient.

If a Method calls for either 100% A or B buffer, then the corresponding port will remain open.

The stir bar in the Gradient Mixer’s mixing chamber will activate whenever the BioLogic LP pump

is running.

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Sommario

Pagina 1 - I Use with the BioLogic LP

1The Gradient Mixer proportions two buffers as commanded by either the BioLogic LP Controller or theEcono Gradient Pump and mixes the buffers in a dyn

Pagina 2

2Figure 2. Use with the BioLogic LPCare of the Gradient Mixer’s Proportioning ValveNormally, the only maintenance required is to flush the valve with

Pagina 3

3To reassemble the unit:1.Wipe the mixing chamber and threads clean.2.Insert the stir bar back into the mixing chamber with the flat end of the stir b

Pagina 4

4II Use with the Econo Gradient PumpPlumbing and Operation of the Gradient Mixer1.To attach the Gradient Mixer to the system rack:a.Ensure that the c

Pagina 5 - 4006069 Rev D

54006069 Rev DBio-Rad Laboratories, 2000 Alfred Novel Drive, Hercules CA 94547Care of the Gradient Mixer’s Proportioning ValveNormally, the only mai