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Appendix

Percent Control Assay Tutorial

1 Defining the Template

2 Setting up the Equations

3 Setting up the Screening Report

4 Entering Experimental Info

5 Setting up the Report

An exam

ple of how to set up

an ELISA Assay that uses a control and equations to

transform the absorbance data into percentage values of the control well (CNTRL) is

given here. This assay has no standards and no standard curve.

Open sample data file (Percent Control.mpl) with its protocol and template.

C:\Program Files\Bio-Rad\Microplate Manager 6\Sample Data

1 Defining the Template

1. Click on the Template icon in the Main Toolbar

• Blanks are defined as #. The average of these # wells will be subtracted

from all the wells

• BLK are a second reagent blank. The average of these BLK wells will be

subtracted from all the wells.

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Bio-rad Microplate Manager Software Manuale Utente Pagina 90