Bio-rad TC20™ Automated Cell Counter Manuale Utente

Catalog #145-0101TC20™ Automated Cell CounterInstruction Manual

LCD screenKeys for navigationUSB ports A and BSlide portPower switchFig. 1. TC20 cell counter.Fig. 2. Home screen.3 IntroductionUser Interface The use

Fig. 3. Options screen.4IntroductionOptions — for selecting additional operationsOptions Screen The Options screen (Figure 3) contains the following c

Fig. 4. Counting slides and trypan blue dye.Fig. 5. Accessories for the TC20 cell counter.5IntroductionTrypan Blue Dye The Trypan blue dye has been sp

Chapter 2 — Setting Up the TC20™ Automated Cell Counter2

Fig. 6. Setting the date and time.8Setting Up the TC20 Automated Cell CounterSystem ComponentsThe TC20 automated cell counter (catalog #145-0102) incl

95. Finally, when you have made all the modifications use the up or down arrow key to reach the Yes or No options for the Save date and time? setting.

4. Finally, when you have made all the modifications use the up or down key to reach Yes or No options for the Save changes? setting. 5. Press Ente

3Chapter 3 — Performing Cell Counts

Fig. 9. Loading the counting slide.12Performing Cell CountsPerforming Cell CountsLoading SlidesHandle the TC20™ counting slides using the edges and av

Fig. 10. Inserting the counting slide into the TC20 cell counter.Fig. 11. Cell count screen without trypan blue dye.Fig. 12. Cell count screen with tr

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14Counting Samples with Multiple Cell PopulationsWhen counting samples with multiple cell populations that vary greatly in size (for example, fibroblas

15If automatic name serialization is enabled, you can select a name consisting of maximum six characters, the last two fields are reserved for automati

Fig. 15. Image of cells.16Performing Cell CountsExporting the Image of Cells from the Current CountThe image from the most recent count can be downloa

17Performing Cell CountsFig. 16. Histogram for total cell count (sample without trypan blue dye).Fig. 17. Histogram for live and dead cell count (sam

Chapter 4 — Saved Count Results4

Fig. 18. Previous counts results.20Saved Count ResultsSaved Count ResultsViewing Previous CountsThe TC20™ cell counter stores results from 100 reads,

21Saved Count Results2. Use the down key to select Export previous counts, and press Enter. If the USB flash drive is already inserted into instrum

22Deleting All Counts To delete all counts stored in the instrument:1. From the Home screen, use the down arrow key to select Options, and press Enter

Chapter 5 — Maintenance and Troubleshooting5

Fig. 19. Disabling user defined gates.Fig. 20. System verification results.24Maintenance and TroubleshootingMaintenance and TroubleshootingPerforming th

TC20™ AutomatedCell CounterInstruction Manual

Fig. 19. Disabling user defined gates.Fig. 20. System verification results.25Maintenance and TroubleshootingCleaning the TC20 Automated Cell CounterThe

26Maintenance and TroubleshootingTo load a new roll of labels:1. Turn the printer on. 2. Open the cover.3. Place the new roll as directed, with the fir

27 Maintenance and TroubleshootingTroubleshootingError Message Possible Cause and Solution Invalid result data Data were improperly stored and can

28Ordering InformationCatalog # DescriptionTC10 Automated Cell Counter145 -0102 TC20 Automated Cell Counter, 120–240 V, includes instrument, power s

Life ScienceGroup13-1888 1213 Sig 121310024423 Rev B US/EGBio-Rad Laboratories, Inc.Web site www.bio-rad.com USA 800 424 6723 Australia 61

iiBio-Rad Laboratories ResourcesTable 1 lists Bio-Rad resources and how to locate what you need. Table 1. Bio-Rad resources.Resource How to Con

iiiSafety ComplianceThis instrument has been tested and found to be in compliance with all applicable requirements of the following safety and electro

ivBio-Rad Laboratories is not responsible for any injury or damage caused by the use of this instrument for purposes other than those for which it is

vTable of ContentsBio-Rad Laboratories Resources ...iiWarranty ...

Chapter 1 — Introduction1

2IntroductionIntroductionThe Bio-Rad TC20™ automated cell counter is a benchtop instrument that counts mammalian cells in one simple step, initiating