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Analyzing Data Using Cutoffs

Data transformed – viewed as a Percent (0 - 100 %)

• Low Cutoff: 0

• High Cutoff: 100

Using values from a Standard Curve – standards 0, 5, 10, 25, 50, 100, 250, and 500

defined on template

• Low Cutoff: ! 0

• High Cutoff: ! 500

Using an equation – sample (SAMPLE2) well(s) defined on template

• Low Cutoff: 0

• High Cutoff: (SAMPLE2-0.025)*100

Setting Up a Percent Control Cutoff

Analysis Report

Define the desired cutoffs in the Cutoff Report Editor window.

For example, the Max High is defined as ≥ 85% of the control in all samples. The

other high and low cutoffs are defined as shown:

Identifiers used in

the Cutoff Report

Editor must be used

on the template.

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Bio-rad Microplate Manager Software Manuale Utente Pagina 56