Bio-rad S3™ Cell Sorter Manuale Utente Scaricare il pdf (Pagina 66)

56 | S3 and S3e Cell Sorters

Acquisition

The same can be done for the FL2 positive control, though the compensation required is

much less (Figure 60).

As compensation is adjusted, the compensation matrix will be updated. At any time the

compensation matrix can be viewed using the button on the toolbar (Figure 61).

Fig. 60. Same data plots showing incorrectly compensated ﬁle (left) and correctly

compensated ﬁle (right).

Fig. 59. Density plot showing uncompensated (left) and compensated data (right).

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Cell Sorters 1
Legal Notices 3
Bio-Rad Technical Support 3
Safety Warning Labels 5
Instrument Warning Labels 5
Regulatory Compliance 6
Biohazards 6
Table of Contents 9
Introduction 11
1.1 System Components 12
1.2 Installation Requirements 12
Hardware Description 13
2.1.1 Instrument Back Panel 14
2.1.2 Aerosol Evacuation Port 14
2.2 Fluidics System 15
2.2.2 Loading Stage 16
Instruction Manual 17
2.2.3 Nozzle 18
2.2.4 Sort Collection Chamber 18
2.3 Optics 19
2.4 Electronics 21
Software 23
3.1 Main Software Window 24
3.1.1 Title Bar Buttons 25
3.1.2 File Menu 25
3.1.3 Home Tab Toolbar 26
3.2 Control Panel 30
3.3 Administrator Tab Toolbar 32
3.3.1 Editing QC Criteria 33
3.3.2 Edit Droplets 34
3.3.3 Edit Streams 34
Instruction Manual 35
Test Pattern highlighted 35
3.3.4 User Reports 36
3.3.5 Managing Users 37
3.3.6 Decontaminate 38
3.3.8 Global Preferences 39
Boost Adjustment 40
Force Cleaning 40
Default Plot Statistics 40
Plot Defaults 40
Parameter Names 41
Default Save Location 41
Emergency Contact Information 41
Getting Started 43
4.2 Logging In 44
4.3 Daily Startup 45
4.4 Quality Control 46
4.4.1 Droplet Setup 47
4.4.2 Side Streams 48
4.4.3 Alignment 49
4.4.5 Drop Delay Maintenance 50
4.4.6 Recalculate Drop Delay 51
4.4.7 QC Criteria 51
4.4.8 QC Report 51
4.5 Protocols and Workspace 52
4.5.3 Saving Protocols 53
4.5.5 Opening FCS Files 54
4.5.6 Saving FCS Files 55
4.5.10 Changing Parameters 56
4.5.11 Viewing Statistics 57
4.5.13 Creating Regions 58
4.5.14 Creating Gates 59
Acquisition 61
5.1.1 Target Event Rate 62
5.1.2 Event Limit 63
5.1.3 Cycle Mode 63
5.1.4 Trigger 63
5.1.7 Auto Save 64
5.1.5 Threshold 64
5.1.6 PMT Voltages 64
5.2 Compensation 65
Instruction Manual 67
Fig. 61. Compensation matrix 67
5.2.2 Manual Compensation 69
5.3.1 Hot Swap 70
Instruction Manual 71
5.4 Optical Filters 72
5.4.2 Optical Filter Blocks 73
6.1 Sort Setup 75
Fig. 70. Sort Logic window 76
6.1.1 Sort Collection 77
6.1.2 Volume Tracker 77
6.1.3 Setting Regions to Sort 78
6.2 Sort Modes 79
6.3 Sort Statistics 79
6.4 Sort Plots 81
Additional Software Features 83
7.4 Clean System 84
7.5 Instrument Status Box 85
7.6 Status Bar 85
7.8 Quality Control Reports 86
7.7 Printing 86
7.9 User Reports 87
7.10 Biosafety System 88
Shutdown 89
Instruction Manual 91
8.1 Daily Shutdown 91
Automatic Startup 93
10.2 Dealing with Clogs 98
Maintenance 100
10.4 Swap Nozzle Tip Wizard 101
Instruction Manual 101
Instruction Manual 103
Instruction Manual 105
Cleaning the nozzle tip: 108
Sonication: 108
Syringe wash: 108
Instruction Manual 109
Instruction Manual 111
Instruction Manual 113
10.5 Optical Filter Cleaning 115
10.6 Disinfectants 115
10.7 Decontamination 115
Instruction Manual 119
Troubleshooting 121
References 125
and S3e 127
Cell Sorter 127
Specifications 127
Ordering Information 129
Life Science 132
Bio-Rad 132
Laboratories, Inc 132

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Bio-rad S3™ Cell Sorter Manuale Utente Pagina 66

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