Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuale Utente Scaricare il pdf (Pagina 22)

Section 5

Care of the Cartridge

5.1 Regeneration

After each use, the cartridge should be regenerated

with the appropriate salt, in most cases 1–2 M NaCl

in the presence of buffer. Wash with 2 to 4 column

volumes of the buffered high salt solution. This

reduces the potential for protein precipitation when

selecting acid as a cleaning agent.

5.2 Cleaning

After repeated use, an ion exchange cartridge may

require thorough cleaning and regeneration to

remove bound contaminants. Acceptable cleaning-

in-place (CIP) reagents include 1% acetic acid/1%

phosphoric acid with 0.4 M NaCl, up to 30% acetic

acid, 1% Triton X-100, up to 70% ethanol or 30%

isopropyl alcohol, 8 M urea, and 6 M guanidine-HCl.

Any of these agents can be combined in an

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Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuale Utente Pagina 22