Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuale Utente Scaricare il pdf (Pagina 17)

Section 4

Preparing a Cartridge

For Use

Bio-Scale Mini Macro-Prep High Q, Macro-Prep

High S, and Macro-Prep DEAE cartridges contain

20% ethanol (v/v) as the storage solution. The fully

hydrated support is ready to use after equilibrating

the cartridge in the buffer of choice. To perform

buffer exchange, connect the cartridge to a liquid

chromatography system or peristaltic pump and

condition it as instructed below:

1. Set pump flow rate to 3.0 ml/min (731 cm/hr)

for the 1 ml cartridge or 6.0 ml/min (288 cm/hr)

for the 5 ml cartridge.

2. Wash the cartridge with degassed low-salt

buffer for 2 min.

3. Wash the cartridge with degassed high-salt

buffer for 5 min.

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Bio-rad Bio-Scale™ Mini Macro-Prep® High Q and Macro-Prep Manuale Utente Pagina 17