Bio-rad Aurum™ Plasmid Mini Purification Module Manuale Utente

Bio-Rad Laboratories, Inc.2000 Alfred Nobel Dr.Hercules, CA 94547 USA(510) 741-10001-800-424-67234110111 Rev C

7Note: The neutralization solution should be added within 5 min after lysis.4. Add 350 µl of neutralization solution and mix by inverting the capped t

8Fig. 5. Aurum plasmid mini protocol overview: vacuum formatGrowth and Isolation 1. Grow 1–5 ml bacterial culture overnight or16 hr.2. Measure A600(i

9Spin FormatThe Aurum plasmid mini kit can be used with any commercially availablemicrocentrifuge that can accommodate 1.5 ml and 2.0 ml tubes. All ce

10Fig. 6. Aurum plasmid mini protocol overview: spin formatGrowth and Isolation1. Grow 1–5 ml bacterial culture overnight or16 hr.2. Measure A600(if

11Section 7Troubleshooting Guide Problem Possible Cause Possible SolutionLow plasmid yields Low copy number Use high copy numberplasmid constructsPoo

12Problem Possible Cause Possible SolutionBacterial DNA Excessive amount of Determine OD600of contamination bacteria processed culture and do notexcee

13Problem Possible Cause Possible SolutionMore than one band Presence of multimers – Try different host or on analytical gel typical and variable grow

Aurum™Plasmid Mini Kit Instruction ManualFor technical service, call your local Bio-Rad office, orin the US, call 1-800-424-6723

Table of ContentsSection 1 Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . .1Section 2 Kit Components . . . . . . . . . . . . .

Section 1Introduction The new Aurum plasmid mini kit is optimized for the purification of up to 20 µgof plasmid DNA, rapidly and inexpensively, withou

Section 3Storage Conditions Solutions and columns should be stored at room temperature. Ifprecipitation is observed in any solution, warm solution to

Section 5Guidelines for Using the AurumPlasmid Mini Kit Please read the following guidelines before starting the plasmid purification.Bacterial Growth

media when 10–12 OD•ml of bacteria are processed per column, althoughsmaller amounts of culture may also be processed.To determine the density of a ba

Section 6Protocol Vacuum FormatThis procedure requires the Bio-Rad Aurum vacuum manifold and columnadaptor plate (Cat. #732-6470), or any vacuum manif

6Fig. 3a. Vacuum setupconditions for the Aurumplasmid mini kit Fig. 3b. Connection of Aurummini column to column adaptorplate Aurum mini columnColu