Bio-rad PV92 PCR Informatics Kit Manuale Utente Scaricare il pdf (Pagina 3)

Quick Guide

1. Label 1 screwcap tube containing 200 µl of

InstaGene matrix plus protease with your

initials.

2. Collect 2 hairs from yourself. Select hairs that

have either a noticeable sheath (a coating of

epithelial cells near the base of the hair), or a

good sized root (the bulb-shaped base of the

hair). Trim the hair, leaving the last ~2 cm of the

base of the hair. Place the trimmed hairs into

the screwcap tube with your initials.

3. Place your tube in the foam micro test-tube

holder and incubate at 56°C for 10 minutes in

a water bath. At the halfway point (5 minutes),

shake or vortex gently, then place it back in the

56°C water bath for the remaining 5 minutes.

4. Remove the tubes, gently shake or vortex, and

place them in a boiling water bath (100°C).

Incubate at 100°C for 5 minutes.

5. Remove the tubes from the boiling water bath

and shake or vortex the contents to resuspend.

Pellet the matrix by spinning at 6,000 x g for

5 minutes (or 2,000 x g for 10 minutes) in a

centrifuge.

6. Store your screwcap tube in the refrigerator until

the next laboratory period (or proceed to step 2

of Lesson 2).

Centrifuge

56°C, 10 min

Sheath

Bulb

Water bath

100°C, 5 min

Water bath

Lesson 1 Hair Follicle DNA Template Preparation

1 2 3 4 5 6 7

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Bio-rad PV92 PCR Informatics Kit Manuale Utente Pagina 3