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1. Label one 1.5 ml micro test tube with your

initials. Label one screwcap tube containing

200 µl of InstaGene matrix with your initials.

2. Obtain a cup containing saline solution from

your instructor. Pour the saline into your mouth

and rinse vigorously for 30 seconds. Expel the

saline back into the cup.

3. Transfer 1 ml of your saline rinse into the micro

test tube (NOT the screwcap tube) with your

initials. If a P-1000 micropipet is not available,

carefully pour ~1 ml of your saline rinse into

your micro test tube (use the graduations on the

side of the micro test tube to estimate 1 ml).

4. Spin your tube in a balanced centrifuge at full

speed for 2 minutes. When the centrifuge has

completely stopped, remove your tube. You

should see a match-head sized pellet of whitish

cells at the bottom of the tube. If you don’t see

a pellet of this size, decant the saline, refill your

tube with more of your oral rinse, and repeat the

spin.

5. After pelleting your cells, pour off the saline.

Being careful not to lose your pellet, blot your

tube briefly on a paper towel or tissue. It’s OK

for a small amount of saline (< 50 µl, about the

same size as your pellet) to remain in the

bottom of the tube.

6. Resuspend the pellet by vortexing or flicking the

tube so that no clumps of cells remain.

7. Using a 2–20 µl adjustable-volume micropipet

set to 20 µl, transfer all of your resuspended

cells to the screwcap tube containing

InstaGene.

8. Screw the cap tightly on the tube. Shake or

vortex to mix the tube contents.

Quick Guide

Lesson 1 Cheek Cell DNA Template Preparation

Centrifuge

1 2 3 4 5 6 7

Sommario

Pagina 1 - Quick Guide

1. Label one 1.5 ml micro test tube with your initials. Label one screwcap tube containing 200 µl of InstaGene matrix with your initials. 2. Obtain a

Pagina 2 - Water bath

9. When all members of your team have collected their samples, place the tubes in thefoam micro test-tube holder, and incubate at56°C for 10 minutes i

Pagina 3

Quick Guide 1. Label 1 screwcap tube containing 200 µl ofInstaGene matrix plus protease with your initials.2. Collect 2 hairs from yourself. Select ha

Pagina 4

Quick GuideLesson 2 PCR Amplification1. Obtain the tube with your DNA template fromthe refrigerator. Spin the screwcap tube for 2minutes at 6,000 x

Pagina 5 - Lane Sample Load Volume

Lesson 3 Electrophoresis of AmplifiedPCR Samples and Staining of AgaroseGels1. Obtain your PCR tube from the thermal cyclerand place in the capless

Pagina 6

2. There are two protocols for staining your gel.Your instructor will inform you which one youwill use.Protocol 1: Quick staining (requires 12–15 minu

Pagina 7

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