Bio-rad Ligation and Transformation Module Manuale Utente Scaricare il pdf (Pagina 3)

Table of Contents

Introduction ..................................................................................1

Kit Inventory Checklist ................................................................6

Safety Issues ................................................................................9

Background ................................................................................10

Quick Guide ................................................................................26

Instructor’s Advance Preparation ............................................32

Student Ligation Protocol..........................................................36

Student Transformation Protocol..............................................40

Appendix A Inoculating a Bacterial Colony for

Plasmid Miniprep ................................................46

Appendix B Restriction Digestion of Plasmid DNA

with Bgl II Enzyme ..............................................48

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Bio-rad Ligation and Transformation Module Manuale Utente Pagina 3