Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuale Utente Scaricare il pdf (Pagina 15)

ENrich Instruction Manual 11

7. Stop the pump. Plug the bottom of the column. Set the

column upright in a beaker. Remove the tubing and ﬁtting

from the top of the adaptor.

8. Remove the frit from the adaptor by hooking one end of the

frit removal tool/tweezer into the frit in a sideways motion

with slight downward pressure.

9. Push the new frit into the end of the adaptor.

10. Replace the O-rings if they appear worn or torn. If the

O-rings are replaced, wet them with buffer before the next

step.

11. Add a few drops of buffer to the top of the resin bed. Insert

the adaptor and push it down to the bed. Some buffer

should ﬂow out of the top of the column.

12. Screw on the upper retainer (Figure 7).

Fig. 7. Reattach and hand-tighten the retainer.

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Bio-rad ENrich™ High-Resolution Ion Exchange Columns Manuale Utente Pagina 15