Bio-rad Chromatography Standards Manuale Utente Scaricare il pdf (Pagina 6)

Fig. 1.

a. Bio-Sil SEC 125 column (exclusion limit* ≈ 80,000).

b. Bio-Sil SEC 250 column (exclusion limit ≈ 300,000).

c. Bio-Sil SEC 400 column (exclusion limit ≈ 800,000).

Peaks:

A. Protein aggregates (void peak)

B.Thyroglobulin

C.γ-globulin

D.Ovalbumin

E. Myoglobin

F. Vitamin B

Conditions

Buffer 0.05 M Na

HPO

, 0.05 M NaH

0.15 M NaCl, pH 6.8

Column 300 x 7.8 mm

Flow rate 1.0 ml/min

Sample 20 µl

Chart speed 1 cm/min

Detection UV @ 280 nm; 1.0 AUFS

*Nominal exclusion limits given for globular proteins.

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Bio-rad Chromatography Standards Manuale Utente Pagina 6