Bio-rad Bio-Scale™ MT High-Resolution Empty Columns Manuale Utente Scaricare il pdf (Pagina 9)

Bio-Scale MT Column Instruction Manual 5

9. Gently turn the lock nut until the upper bed support is in the

desired position.

10. Attach upper (1) and lower (11) end ﬁttings.

2.3 Column Packing

Always follow the manufacturer’s instructions for packing a

particular type of media. Generally, slurry packing will give better

chromatographic performance than dry packing. The following

guidelines may be useful:

Dry packing Particle size Ž 20 µm

Slurry packing Particle size < 20 µm

Dry Packing

Assemble the column according to the following ﬁgure.

Fig. 2 Column tube assembly.

1. Holding the column tube vertically, ﬁll one half with the packing

media.

2. Tap the side of the column until the media settles.

3. Fill the column with the remainder of the media and repeat

step 2.

4. When the column is ﬁlled to the desired level, complete the

column assembly as described in Section 2.2, making sure the

ﬂow direction arrow on the glass tube is pointing in the current

direction.

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Bio-rad Bio-Scale™ MT High-Resolution Empty Columns Manuale Utente Pagina 9