Bio-rad Bio-Scale™ CHT™ Type I Columns Manuale Utente Scaricare il pdf (Pagina 2)

Table of Contents

Page

Section 1 Characteristics of the Bio-Scale CHT2, CHT5, CHT10, CHT20

Ceramic Hydroxyapatite, Type I Columns...............................................1

1.1 Introduction ................................................................................................................1

1.2 The CHT-I Separation Medium.................................................................................1

1.3 Connection to the BioLogic, FPLC

and HPLC Systems ........................................1

Section 2 Use of the Bio-Scale CHT-I Columns.........................................................2

2.1 Preparation for Initial Use..........................................................................................2

2.2 Sample Preparation ....................................................................................................3

2.3 Elution Conditions......................................................................................................3

2.4 Use of Detergents.......................................................................................................4

2.5 Effect of Calcium Chloride........................................................................................4

2.6 Chromatography Pre-Runs.........................................................................................4

Section 3 Care of the Bio-Scale CHT-I Columns ......................................................5

3.1 Column Hygiene ........................................................................................................5

3.2 Bed Height Adjustment..............................................................................................5

3.3 Frit Removal...............................................................................................................5

3.4 Top-Off Resin.............................................................................................................5

3.5 Storage Conditions.....................................................................................................5

3.6 References ..................................................................................................................8

Section 4 Product Information....................................................................................8

1 2 3 4 5 6 7 ... 10 11

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Bio-rad Bio-Scale™ CHT™ Type I Columns Manuale Utente Pagina 2